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验证数据展示

  • WB analysis of rat liver using 85185-4-RR

    WB analysis of rat liver using 85185-4-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 85185-4-RR (HSCB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HSCB Recombinant antibody

HSCB Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85185-4-RR

Print datasheet

CloneNo. 242836H3

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Iron-sulfur cluster co-chaperone protein HscB, mitochondrial, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic, Iron-sulfur cluster co-chaperone protein HscB, HSC20, DNAJC20

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inrat liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85185-4-RR targets HSCB in WB, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, rat
Immunogen HSCB fusion protein Ag7268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HscB iron-sulfur cluster co-chaperone homolog (E. coli)
Synonyms Iron-sulfur cluster co-chaperone protein HscB, mitochondrial, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic, Iron-sulfur cluster co-chaperone protein HscB, HSC20, DNAJC20
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC065569
Gene Symbol HSCB
Gene ID (NCBI) 150274
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8IWL3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSCB, also named as HSC20 and DNAJC20, acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria.

实验方案

Product Specific Protocols
WB protocol for HSCB antibody 85185-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols