• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 85360-1-RR

    WB analysis using 85360-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85360-1-RR (SYN1-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse retina using 85360-1-RR

    IF Staining of mouse retina using 85360-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse retina tissue using SYN1-Specific antibody (85360-1-RR, Clone: 242415H5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SYN1-Specific Recombinant antibody

SYN1-Specific Uni-rAbTM Recombinant Antibody for WB, IF-P, ELISA
Cat No. 85360-1-RR

产品说明书

CloneNo. 242415H5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

SYN1, Synapsin I, 242415H5, Brain protein 4.1, SYN1a

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inIMR-32 cells, SH-SY5Y cells, SK-N-SH cells
Positive IF-P detected inmouse retina tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85360-1-RR targets SYN1-Specific in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 synapsin I
别名 SYN1, Synapsin I, 242415H5, Brain protein 4.1, SYN1a
计算分子量 74 kDa
观测分子量 70-80 kDa
GenBank蛋白编号NM_006950
基因名称 SYN1
Gene ID (NCBI) 6853
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17600
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SYN1, also named Brain protein 4.1, belongs to the synapsin family. SYN1 is a neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level. Defects in SYN1 are a cause of epilepsy X-linked with variable learning disabilities and behavior disorders (XELBD).

实验方案

Product Specific Protocols
WB protocol for SYN1-Specific antibody 85360-1-RRDownload protocol
IF protocol for SYN1-Specific antibody 85360-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols