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Anti-Human TNFR2/CD120b Rabbit Recombinant Antibody, PBS Only

TNFR2/CD120b Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230328B3

Cat No : 98010-1-PBS

Print datasheet

Synonyms

TNFRSF1B, p80 TNF alpha receptor, p75TNFR, p75, Etanercept

验证数据展示

  • FC experiment of human PBMCs using 98010-1-RR (same clone as 98010-1-PBS)

    FC experiment of human PBMCs using 98010-1-RR (same clone as 98010-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TNFR2/CD120b (98010-1-RR, Clone:230328B3) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed. This data was developed using the same antibody clone with 98010-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98010-1-PBS

    FC experiment of human PBMCs using 98010-1-PBS

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TNFR2/CD120b (98010-1-PBS, Clone:230328B3) or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • Cytometric bead array standard curve of MP00777-1

    Cytometric bead array standard curve of MP00777-1

    Cytometric bead array standard curve of MP00777-1, TNFRSF1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83101-2-PBS. Detection antibody: 98010-1-PBS. Standard: Eg0086. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00777-2

    Cytometric bead array standard curve of MP00777-2

    Cytometric bead array standard curve of MP00777-2, TNFRSF1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83101-3-PBS. Detection antibody: 98010-1-PBS. Standard: Eg0086. Range: 0.625-20 ng/mL.

  • Cytometric bead array standard curve of MP00777-3

    Cytometric bead array standard curve of MP00777-3

    Cytometric bead array standard curve of MP00777-3, TNFRSF1B Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83101-4-PBS. Detection antibody: 98010-1-PBS. Standard: Eg0086. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 98010-1-RR

    Affinity and Kinetic Characterization of 98010-1-RR

    Surface Plasmon Resonance (SPR) kinetic assays of 98010-1-RR against Human TNFR2/CD120b were performed. The affinity constant is 7.52 nM.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy TNFR2/TNFRSF1B Ready-To-Use IHC Kit. TNFR2/TNFRSF1B primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98010-1-PBS targets TNFR2/CD120b as part of a matched antibody pair:

MP00777-1: 83101-2-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)

MP00777-2: 83101-3-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)

MP00777-3: 83101-4-PBS capture and 98010-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 1B
Synonyms TNFRSF1B, p80 TNF alpha receptor, p75TNFR, p75, Etanercept
Calculated Molecular Weight 48 kDa
GenBank Accession NumberBC052977
Gene Symbol TNFR2
Gene ID (NCBI) 7133
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20333
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Tumor necrosis factor-alpha (TNFA/TNFSF2) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis (PMID: 16407280). TNFA signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a, p55) and TNFR2 (TNFRSF1B, CD120b, p75). TNFR1 is widely expressed, whereas TNFR2 exhibits more restricted expression, being found on CD4 and CD8 T lymphocytes, endothelial cells, microglia, oligodendrocytes, neuron subtypes, cardiac myocytes, thymocytes and human mesenchymal stem cells (PMID: 20489699; 22374304). In contrast to TNFR1, TNFR2 does not have a death domain. TNFR2 only signals for antiapoptotic reactions. However, recent evidence indicates that TNFR2 also signals to induce TRAF2 degradation (PMID: 22374304). Various defects in the TNFR2 pathway, due to polymorphisms in the TNFR2 gene, upregulated expression of TNFR2 and TNFR2 shedding, have been implicated in the pathology of several autoimmune disorders (PMID: 20489699).

实验方案

Product Specific Protocols
FC protocol for TNFR2/CD120b antibody 98010-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols