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Anti-Human TIGIT Rabbit Recombinant Antibody, PBS Only

TIGIT Uni-rAbTM Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240423A7

Cat no : 98023-3-PBS

Print datasheet

Synonyms

VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains

验证数据展示

  • FC experiment of human PBMCs using 98023-3-RR (same clone as 98023-3-PBS)

    FC experiment of human PBMCs using 98023-3-RR (same clone as 98023-3-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human TIGIT Rabbit Recombinant Antibody (98023-3-RR, Clone: 240423A7) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98023-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00528-3

    Cytometric bead array standard curve of MP00528-3

    Cytometric bead array standard curve of MP00528-3, TIGIT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98023-3-PBS. Detection antibody: 83545-3-PBS. Standard: Eg0749. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 98023-3-RR

    Affinity and Kinetic Characterization of 98023-3-RR

    Biolayer interferometry (BLl) kinetic assays of 98023-3-RR against Human TIGIT were performed. The affinity constant is 4.00 nM.

产品信息

98023-3-PBS targets TIGIT as part of a matched antibody pair:

MP00528-3: 98023-3-PBS capture and 83545-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name T cell immunoreceptor with Ig and ITIM domains
Synonyms VSTM3, VSIG9, V-set and transmembrane domain-containing protein 3, V-set and immunoglobulin domain-containing protein 9, T-cell immunoreceptor with Ig and ITIM domains
Calculated Molecular Weight26 kDa
GenBank Accession NumberNM_173799.4
Gene Symbol TIGIT
Gene ID (NCBI) 201633
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ495A1-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TIGIT (T-cell immunoreceptor with Ig and ITIM domains), also known as VSIG9 or VSTM3, is an immune receptor expressed on T cells, including Treg and memory subsets, as well as on NK cells (PMID: 19011627). It contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT binds to poliovirus receptor (PVR, also called CD155) with high affinity, and also to PVRL2 (CD112) with lower affinity. The interaction of TIGIT with PVR on dendritic cells increases the secretion of IL-10 and decreases the secretion of proinflammatory cytokine and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT can inhibit NK cytotoxicity directly through its ITIM (PMID: 19815499).