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验证数据展示

  • FC experiment of rat splenocytes using 98046-1-RR

    FC experiment of rat splenocytes using 98046-1-RR

    1x10^6 unstimulated or PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with APC Anti-Rat CD4 (OX-35) and 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of rat splenocytes using 98046-1-RR

    FC experiment of rat splenocytes using 98046-1-RR

    1x10^6 PMA, Ionomycin and Brefeldin A treated rat splenocytes were intracellularly stained with 0.25 ug Anti-Rat IL-2 Rabbit Recombinant Antibody (98046-1-RR, Clone: 240264C7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98046-1-RR

    Affinity and Kinetic Characterization of 98046-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98046-1-RR against Rat IL2 were performed. The affinity constant is 0.36 nM.

Anti-Rat IL-2 Rabbit Recombinant Antibody

IL-2 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98046-1-RR

产品说明书

CloneNo. 240264C7

宿主/亚型

Rabbit / IgG

种属反应性

Rat

应用

FC (Intra)

别名

Il2, interleukin 2, 240264C7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated rat splenocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98046-1-RR targets IL-2 in FC (Intra) applications and shows reactivity with Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Rat
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 2
别名 Il2, interleukin 2, 240264C7
计算分子量18 kDa
GenBank蛋白编号NM_053836
基因名称 Il2
Gene ID (NCBI) 116562
RRIDAB_3672193
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP17108
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Stable for one year after shipment.

背景介绍

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of the IL-2Rβ and IL-2Rγ receptor subunits (PMID: 25992858, 24907378, 15034008, 17981641).

实验方案

Product Specific Protocols
FC protocol for IL-2 antibody 98046-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols