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Anti-Human CD79a Rabbit Recombinant Antibody

CD79a Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241299F3

Cat no : 98072-1-RR

Print datasheet

Synonyms

MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain

验证数据展示

  • FC experiment of human PBMCs using 98072-1-RR

    FC experiment of human PBMCs using 98072-1-RR

    1x10^6 human PBMCs were stained with CoraLite® Plus 647 Anti-Human CD19 (HIB19) (CL647-65110, Clone: HIB19) and 0.25 ug Anti-Human CD79a Rabbit Recombinant Antibody (98072-1-RR, Clone:241299F3), or Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using 98072-1-RR

    FC experiment of human PBMCs using 98072-1-RR

    1x10^6 human PBMCs were stained with 0.25 ug Anti-Human CD79a Rabbit Recombinant Antibody (98072-1-RR, Clone:241299F3)(red), or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98072-1-RR

    Affinity and Kinetic Characterization of 98072-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98072-1-RR against Human CD79a were performed. The affinity constant is 0.151 nM.

经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD79a Ready-To-Use IHC Kit. CD79a primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98072-1-RR targets CD79a in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD79a molecule, immunoglobulin-associated alpha
Synonyms MB 1, Ig-alpha, IGA, Ig alpha, B-cell antigen receptor complex-associated protein alpha chain
Calculated Molecular Weight 226 aa, 25 kDa
GenBank Accession NumberBC113733
Gene Symbol CD79A
Gene ID (NCBI) 973
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP11912
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD79a, also named as B-cell antigen receptor complex-associated protein alpha chain or MB-1 membrane glycoprotein, is a 226 amino acid protein, which contains one ITAM domain and one Ig-like C2-type (immunoglobulin-like) domain. CD79A is expressed in B cells and localizes in the cell membrane. CD79A is required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79A is also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells.

实验方案

Product Specific Protocols
FC protocol for CD79a antibody 98072-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols