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验证数据展示

  • FC experiment of human PBMCs using 98096-1-RR

    FC experiment of human PBMCs using 98096-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98096-1-RR

    FC experiment of human PBMCs using 98096-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (98096-1-RR, Clone: 241318H5) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98096-1-RR

    Affinity and Kinetic Characterization of 98096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98096-1-RR against Human BTLA/CD272 were performed. The affinity constant is 1.84 nM.

Anti-Human BTLA/CD272 Rabbit Recombinant Antibody

BTLA/CD272 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98096-1-RR

产品说明书

CloneNo. 241318H5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98096-1-RR targets BTLA/CD272 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B and T lymphocyte associated
别名 BTLA, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, B and T lymphocyte associated, B and T lymphocyte attenuator
计算分子量 289 aa, 33 kDa
GenBank蛋白编号BC107091
基因名称 BTLA
Gene ID (NCBI) 151888
RRIDAB_3672243
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ7Z6A9
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for BTLA/CD272 antibody 98096-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols