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Anti-Human VISTA Rabbit Recombinant Antibody

VISTA Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241003C8

Cat no : 98109-1-RR

Print datasheet

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98109-1-RR

    FC experiment of human peripheral blood leukocytes using 98109-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98109-1-RR

    FC experiment of human peripheral blood leukocytes using 98109-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human VISTA Rabbit Recombinant Antibody (98109-1-RR, Clone:241003C8) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98109-1-RR

    Affinity and Kinetic Characterization of 98109-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98109-1-RR against Human VISTA were performed. The affinity constant is 0.933 nM.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
Planning an IHC experiment? We recommend our IHCeasy VISTA Ready-To-Use IHC Kit. VISTA primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98109-1-RR targets VISTA in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chromosome 10 open reading frame 54
Synonyms C10orf54, SISP1, PP2135, GI24, B7-H5
Calculated Molecular Weight 311 aa, 34 kDa
GenBank Accession NumberBC020568
Gene Symbol VISTA
Gene ID (NCBI) 64115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H7M9
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

实验方案

Product Specific Protocols
FC protocol for VISTA antibody 98109-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols