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Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody

TNFRSF9/CD137 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241344F3

Cat no : 98112-1-RR

Print datasheet

Synonyms

TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor

验证数据展示

  • FC experiment of human PBMCs using 98112-1-RR

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated or Con-A-stimulated human PBMCs were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8 (UCHT4) Mouse IgG2a Recombinant Antibody (APC-65559, Clone: UCHT4). Cells were not fixed.

  • FC experiment of human PBMCs using 98112-1-RR

    FC experiment of human PBMCs using 98112-1-RR

    1x10^6 unstimulated (black) or Con-A-stimulated human PBMCs (red) were surface stained with 0.25 ug Anti-Human TNFRSF9/CD137 Rabbit Recombinant Antibody (98112-1-RR, Clone: 241344F3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), or Isotype Control (blue). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98112-1-RR

    Affinity and Kinetic Characterization of 98112-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98112-1-RR against Human TNFRSF9/CD137 were performed. The affinity constant is 10.3 nM.

经过测试的应用

Positive FC detected inCon-A-stimulated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98112-1-RR targets TNFRSF9/CD137 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 9
Synonyms TNFRSF9, ILA, CD137, 4-1BB ligand receptor, 4 1BB ligand receptor
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC006196
Gene Symbol TNFRSF9/CD137
Gene ID (NCBI) 3604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ07011
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD137, also known as TNFRSF9 or 4-1BB, is an inducible T cell surface receptor that belongs to the tumor necrosis factor receptor superfamily. CD137 is a transmembrane protein expressed on the surface of activated T-cells. In addition, activation-dependent expression of CD137 has also been found in B lymphocytes, monocytes, and diverse nonlymphoid cell types. CD137 provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. (PMID: 9826581; 23696891)

实验方案

Product Specific Protocols
FC protocol for TNFRSF9/CD137 antibody 98112-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols