• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD30/TNFRSF8 Rabbit Recombinant Antibody

CD30/TNFRSF8 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241330A12

Cat no : 98124-1-RR

Print datasheet

Synonyms

CD30, 241330A12, Ki 1 antigen, Ki 1, D1S166E

验证数据展示

  • FC experiment of human PBMCs using 98124-1-RR

    FC experiment of human PBMCs using 98124-1-RR

    1x10^6 PHA-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Rabbit Recombinant Antibody (98124-1-RR, Clone: 241330A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), 0.25 ug or Isotype Control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (blue). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD30 Rabbit Recombinant Antibody (98124-1-RR, Clone: 241330A12) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98124-1-RR

    Affinity and Kinetic Characterization of 98124-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98124-1-RR against Human CD30 were performed. The affinity constant is 0.528 nM.

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98124-1-RR targets CD30/TNFRSF8 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 8
Synonyms CD30, 241330A12, Ki 1 antigen, Ki 1, D1S166E
Calculated Molecular Weight 64 kDa
GenBank Accession NumberNM_001243
Gene Symbol CD30
Gene ID (NCBI) 943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP28908
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579).

实验方案

Product Specific Protocols
FC protocol for CD30/TNFRSF8 antibody 98124-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols