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Anti-Human TWEAKR/CD266 Rabbit Recombinant Antibody

TWEAKR/CD266 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240991C5

Cat no : 98125-1-RR

Print datasheet

Synonyms

CD266, TNFRSF12A, TweakR, FGF inducible 14, DR3LG

验证数据展示

  • FC experiment of HT-1080 using 98125-1-RR

    FC experiment of HT-1080 using 98125-1-RR

    1x10^6 HT-1080 cells were surface stained with 0.25 ug Anti-Human TWEAKR Rabbit Recombinant Antibody (98125-1-RR, Clone: 240991C5) (red) or 0.25 ug Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98125-1-RR

    Affinity and Kinetic Characterization of 98125-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98125-1-RR against Human CD266 were performed. The affinity constant is 0.508 nM.

经过测试的应用

Positive FC detected inHT-1080 cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 98125-1-RR is TWEAKR/CD266 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen TWEAKR/CD266 fusion protein Ag13823 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor receptor superfamily, member 12A
Synonyms CD266, TNFRSF12A, TweakR, FGF inducible 14, DR3LG
Calculated Molecular Weight 129 aa, 14 kDa
GenBank Accession NumberBC002718
Gene Symbol TWEAKR
Gene ID (NCBI) 51330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NP84
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

TWEAKR (also known as CD266, FN14, TNFRSF12A) is a member of the TNF receptor superfamily which is activated by its ligand, the cytokine TWEAK (TNFSF12). TWEAKR is the smallest member of the TNFR superfamily. TweakR was first described as an FGF-inducible gene that played a role in fibroblast adhesion and migration. Subsequently, the induction of TweakR expression by other growth factors and/or upon tissue injury was observed in multiple cell types, including hepatocytes, endothelial cells, adipocytes, and cardiomyocytes. (PMID: 23073510, PMID: 24409185)

实验方案

Product Specific Protocols
FC protocol for TWEAKR/CD266 antibody 98125-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols