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Recombinant Human Galectin-9/LGALS9 protein (hFc Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

3965

Accession

O00182-2

Cat No : Eg0084

Print datasheet

Synonyms

Galectin 9,LGALS9,Ecalectin,Gal 9,Gal-9

验证数据展示

  • Purity of Recombinant Human Galectin-9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Galectin-9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human Galectin-9 protein Ala2-Thr323 (Accession# O00182-2) with a human IgG1 Fc tag at the N-terminus.
Predicted Molecular Mass 61.8 kDa
SDS-PAGE 68-85 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

References:

1. Barondes SH, et al.(1994). Cell. 25;76(4):597-8.
2. O Türeci, et al. (1997). J Biol Chem. 272(10):6416-22.
3.  Zhang F, et al.(2009). Mol Biol Rep. 36(5):823-30.
4. Zhu C, et al.(2005). Nat Immunol. 6(12):1245-52.