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Recombinant Human IL-6R alpha protein (Myc Tag, His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

C-Myc, His

生物活性

EC50: 32-130 ng/mL

Cat no : Eg0180

Print datasheet

Synonyms

CD126, IL-6R alpha, gp80, IL 6 receptor subunit alpha, IL 6R 1

验证数据展示

  • Purity of Recombinant Human IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-6R alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human IL-6 (Myc tag, His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 32-130 ng/mL.

    Immobilized Human IL-6 (Myc tag, His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 32-130 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human IL-6 (Myc tag, His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 32-130 ng/mL.
来源 HEK293-derived Human IL-6R alpha protein Leu20-Pro365 (Accession# P08887) with a Myc tag and a His tag at the C-terminus.
基因ID 3570
蛋白编号 P08887
预测分子量 41 kDa
SDS-PAGE 60-75 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Interleukin 6 receptor (IL6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. Interleukin 6 (IL6) is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in immune response. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines.

参考文献:

1.	Arnold P., et al. (2020) Cells. 24;9(5):1307.
2.	Yamasaki K., et al. (2020) Neuropsychopharmacol Rep. 40(2):130-137.
3.	Hoek KS., et al. (2008) Pigment Cell Melanoma Res. 21(6):665-76.