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Recombinant Human PCSK9 protein (Myc Tag, His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

Myc Tag, His Tag

生物活性

EC50: 3-12 ng/mL

Cat no : Eg0199

Print datasheet

Synonyms

PCSK9, EC:3.4.21.-, FH3, HCHOLA3, LDLCQ1

验证数据展示

  • Purity of Recombinant Human PCSK9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PCSK9 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human PCSK9 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human LDL R (GST tag) with a linear range of 3-12 ng/mL.

    Immobilized Human PCSK9 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human LDL R (GST tag) with a linear range of 3-12 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human PCSK9 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Biotinylated Human LDL R (GST tag) with a linear range of 3-12 ng/mL.
来源 HEK293-derived Human PCSK9 protein Gln31-Gln692 (Accession# Q8NBP7) with a Myc tag and a His tag at the C-terminus.
基因ID 255738
蛋白编号 Q8NBP7
预测分子量 13.8 kDa (Propeptide) and 62.3 kDa (mature chain)
SDS-PAGE 17 kDa (Propeptide) and 65-75 kDa (mature chain), reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial protein governing the circulating levels of low density lipoprotein-cholesterol (LDL-C), by virtue of its pivotal role in the degradation of the LDL receptor (LDLR). PCSK9 is expressed in the kidney and lung. It is synthesized as a 72 kDa immature precursor that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 63 kDa mature protein. The cleaved N-terminal fragment remains associated with the mature protein and is necessary for its secretion, allowing it to circulate in the blood. The ability of PCSK9 to regulate a diverse group of cell-surface proteins hinted that it might also be able to influence additional membrane proteins that are important in anti-tumour immune responses. Targeting PCSK9 to treat cancer is also attractive because two neutralizing antibodies against it, evolocumab and alirocumab, have already been approved for human clinical use to lower cholesterol levels.

参考文献:

1. Blanchard V, et al. (2019) Pathology. Feb;51(2):177-183.
2. Sharotri V, et al. (2012) J Biol Chem. Jun 1;287(23):19266-74
3. Canuel M, et al. (2013) PLoS One. May 13;8(5):e64145.