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Recombinant Mouse VEGFA protein (His Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0277

Print datasheet

Synonyms

Vegfa, L-VEGF , N-VEGF, Vascular Endothelial Growth Factor, vascular endothelial growth factor A

验证数据展示

  • Purity of Recombinant Mouse VEGFA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse VEGFA was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Mouse VEGFA protein Ala27-Arg190 (Accession# Q00731-2) with a His tag at the N-terminus.
基因ID 22339
蛋白编号 Q00731-2
预测分子量 20.1 kDa
SDS-PAGE 22-26 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). Alternately spliced isoforms of 121, 145, 148, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 144, 164 and 188 aa isoforms found in rat and mouse.

参考文献:

1. Conn, G. et al. (1990) J. Biol. Chem. 87:2628.
2. Ishii, H. et al. (2001) Arch. Oral Biol. 46:77.
3. Byrne, A.M. et al. (2005) J. Cell. Mol. Med. 9:777.
4. Robinson, C.J. and Stringer, S.E. (2001) J. Cell. Sci. 114:853.