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Recombinant Human MMP-3 protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0382

Print datasheet

Synonyms

MMP3, EC:3.4.24.17, Matrix metalloproteinase-3, MMP 3, MMP-3

验证数据展示

  • Purity of Recombinant Human MMP-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human MMP-3 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human MMP-3 protein Tyr18-Cys477 (Accession# P08254) with a His tag at the C-terminus.
基因ID 4314
蛋白编号 P08254
预测分子量 56 kDa
SDS-PAGE 55-65 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

MMP-3 (matrix metallopeptidase 3), also named as Stromelysin 1 or STR1, is a member of matrix metalloproteinase (MMP) family. The MMP family of enzymes is comprised of critically important extracellular matrix remodeling proteases whose activity has been implicated in normal embryogenesis and tissue remodelling and in many diseases such as arthritis, cancer, periodontitis, glomerulonephritis, encephalomyelitis, atherosclerosis and tissue ulceration. These proteases have come to represent important therapeutic and diagnostic targets for the treatment and detection of human cancers. MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fbronectin, laminin, IGFBP-3, serpins, and IL-1β.

参考文献:

1. Roy R. et al. (2009) Journal of Clinical Oncology. 27(31):5287-5297.
2. Nagase H. et al. (1994) Contributions to Nephrology. 107:85.
3. Stamenkovic I. et al. (2003) The Journal of Pathology. 200(4):448-464.
4. Pytliak M. et al. (2012) Onkologie. 35(1-2):49-53.
5. Begoña Arza. et al. (2000). Febs Journal. 267(21):6378.
6. Fan. et al. (2011) International Journal of Molecular Medicine. 27(4).