Recombinant Human MUC17 protein (His Tag)
ED50
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Species
Human
Purity
>90 %, SDS-PAGE
GeneID
140453
Accession
Q685J3-1
验证数据展示
Technical Specifications
Purity | >90 %, SDS-PAGE |
Endotoxin Level | <1.0 EU/μg protein, LAL method |
Biological Activity |
Not tested |
Source | HEK293-derived Human MUC17 protein Arg4131-Leu4390 (Accession# Q685J3-1) with a His tag at the C-terminus. |
Predicted Molecular Mass | 12.6 kDa and 20.5 kDa |
SDS-PAGE | 17 kDa and 28-38 kDa, reducing (R) conditions |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization. |
Reconstitution | Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water. |
Storage |
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature. |
Background
MUC17 is a membrane-bound mucin that provides protection to gut epithelial cells. MUC17 contains about 60 tandem repeats, with each repeat being around 60 aa.Membrane mucins, such as MUC17, function in epithelial cells to provide cytoprotection, maintain luminal structure, provide signal transduction, and confer antiadhesive properties upon cancer cells that lose their apical/basal polarization.
References:
1. Gum JR Jr, Crawley SC, Hicks JW, Szymkowski DE, Kim YS. MUC17, a novel membrane-tethered mucin. Biochem Biophys Res Commun. 2002;291(3):466-475. 2. Pelaseyed T, Bergström JH, Gustafsson JK, et al. The mucus and mucins of the goblet cells and enterocytes provide the first defense line of the gastrointestinal tract and interact with the immune system. Immunol Rev. 2014;260(1):8-20.