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Recombinant Human EMMPRIN/CD147 protein (His Tag)

ED50

/

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

682

Accession

P35613-1

Cat No : Eg0436

Print datasheet

Synonyms

CD147,5F7,Basigin,BSG,Collagenase stimulatory fact

验证数据展示

  • Purity of Recombinant Human EMMPRIN was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human EMMPRIN was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human EMMPRIN protein Glu138-Ala323 (Accession# P35613-1) with a His tag at the C-terminus.
Predicted Molecular Mass 24.5 kDa
SDS-PAGE 32-45 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD147, also known as Basigin or extracellular matrix metalloproteinase inducer (EMMPRIN), is a transmembrane glycoprotein that belongs to the immunoglobulin superfamily. The molecule is composed of an intracellular portion, an extracellular portion, and a single transmembrane region. CD147 is expressed on a variety of cell types (e.g., hematopoietic, epithelial, and endothelial cells) and at varying levels. Increased expression of CD147 occurs in many tumors. CD147 is a pleiotropic molecule that plays an important role in fetal, neuronal, lymphocyte and extracellular matrix development. CD147 has been identified as a receptor essential for erythrocyte invasion by Plasmodium falciparum (PMID: 22080952). It has been reported that spike protein of SARS-CoV-2 binds to CD147 on host cells, thereby mediating the viral invasion.

References:

1. C Biswas, et al. (1995) Cancer Res. 55(2):434-9. 
2. Kathryn T Iacono, et al. (2007) Exp Mol Pathol. 83(3):283-95.
3. Hsiang-Chi Tseng, et al. (2020) Nat Commun. 11(1):4810.
4. Ke Wang, et al. (2020) Signal Transduct Target Ther. 5(1):283.