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Recombinant Human APOE protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0448

Print datasheet

Synonyms

APOE, Apo E, Apo-E, apoe4, apolipoprotein E

验证数据展示

  • Purity of Recombinant Human APOE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human APOE was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human APOE protein Lys19-His317 (Accession# P02649) with a His tag at the C-terminus.
基因ID 348
蛋白编号 P02649
预测分子量 38 kDa
SDS-PAGE 34-36 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Apolipoprotein E (APOE) is a core component of plasma lipoproteins and is involved in a variety of biological processes, including plasma lipoprotein metabolism, intracellular cholesterol utilization, cell growth, immune regulation, and neuronal growth and repair.The ε4 allele of the APOE gene is the strongest genetic risk factor for late-onset Alzheimer's disease (AD). Mouse APOE, like ApoE4, contains equivalents of Arg-112 and Glu-255 but lacks the critical Arg-61 equivalent. APOE has heparin-binding activity and binds to heparan sulfate proteoglycans on the cell surface, a property that facilitates cellular capture and receptor-mediated uptake of APOE-containing lipoproteins.

参考文献:

1. Teng E.,et al. (2015). Dement Geriatr Cogn Disord.39(3-4):154-66.
2. Raffai R.L.,et al. (2001). Proc Natl Acad Sci U S A.98(20):11587-11591.
3. Mooijaart SP,et al. (2006). PLoS Med.3(6):e176.
4. Maloney B,et al. (2007).J Neurochem.103(3):1237-1257.