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Recombinant Human MMP-1 protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0527

Print datasheet

Synonyms

MMP1, 22 kDa interstitial collagenase, 27 kDa interstitial collagenase, CLG, CLGN

验证数据展示

  • Purity of Recombinant Human MMP-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human MMP-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human MMP-1 protein Phe20-Asn469 (Accession# P03956) with a His tag at the C-terminus.
基因ID 4312
蛋白编号 P03956
预测分子量 55.9 kDa
SDS-PAGE 55-65 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Matrix metalloproteinase 1 (MMP1), which is also known as interstitial collagenase and fibroblast collagenase is a member of the matrix metalloproteinases (MMPs) family . Proteins in this family mainly participate in the breakdown of extracellular matrix both in normal physiological processes and disease processes. Several factors contribute to the expression of MMP1, including endogenous factors, such as polymorphisms and epigenetic regulation in the promoter region of MMP1, as well as exogenous factors, such as the tumor microenvironment. A variety of MMP1 promoter genotypic polymorphisms have been reported in various ethnic populations, and their contributions to different disease risks have been explored. In the tumor microenvironment, a variety of inflammatory factors, including interleukin-8 (IL-8), IL-1β and tumor necrosis factor-α (TNF-α), have been reported to induce MMP1 expression in cancer cells.

参考文献:

1. Shen CJ. et al. (2017) PLoS One. 12(3):e0174487. 
2. Chen Y. et al. (2019) Int J Oncol. 55(1):142-156. 
3. Young-Min SA. et al. (2001) Ann Rheum Dis. 60(9):846-51. 
4. Murawaki Y. et al. (1999) J Gastroenterol Hepatol. 14(2):138-45.