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Recombinant Human TNFR1/CD120a protein (His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 0.8-3.2 ng/mL

Cat no : Eg0531

Print datasheet

Synonyms

CD120a, TNFR1, TNFRSF1A, FPF, p55

验证数据展示

  • Purity of Recombinant Human TNFR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TNFR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.

    Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.
来源 HEK293-derived Human TNFR1 protein Ile22-Thr211 (Accession# P19438-1) with a His tag at the C-terminus.
基因ID 7132
蛋白编号 P19438-1
预测分子量 22.1 kDa
SDS-PAGE 32-48 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55 kDa TNFR1. Soluble TNFR1 (sTNFR1) could function as TNF-binding protein, inhibiting TNF bioactivity.

参考文献:

1.  Islam A, et al. (2006). J Biol Chem. 281(10):6860-73.
2.  Aggarwal BB, et al. (2012). Blood. 119(3):651-65.
3.  Aderka D , et al. (2012). J Exp Med. 175(2):323-9.