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Recombinant Human AFP/Alpha-fetoprotein protein (His Tag)

ED50

/

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

174

Accession

P02771

Cat No : Eg0539

Print datasheet

Synonyms

AFP, Alpha 1 fetoprotein, alpha fetoprotein, FETA, HPAFP

验证数据展示

  • Purity of Recombinant Human AFP/Alpha-fetoprotein was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human AFP/Alpha-fetoprotein was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
/
Source HEK293-derived Human AFP/Alpha-fetoprotein protein Arg19-Val609 (Accession# P02771) with a His tag at the C-terminus.
Predicted Molecular Mass 68.3 kDa
SDS-PAGE 67-80 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

AFP (alpha-fetoprotein) is a major plasma protein found in the fetus while plasma AFP level is decreased rapidly after birth. AFP is a vital marker of the hepatocyte lineage. High AFP concentrations is related with tumor cell growth. Detection of AFP in plasma is important in diagnosis of hepatocellular carcinoma (HCC), stomach cancer and germ cell cancers. Altered levels of both fetal and maternal AFP are associated with hypothyroidism, autoimmune disorders, and heart defects. Measurement of AFP in maternal blood or amniotic fluid of pregnant women is used in screening developmental abnormalities such as aneuploidy. Serum AFP level is elevated in people with developmental birth defects and neurologic disorders.

References:

1. Rosen T and D'Alton ME. Down syndrome screening in the first and second trimesters: what do the data show? Semin Perinatol. 29(6):367-75 (2005).
2. Schieving JH, et al. Alpha-fetoprotein, a fascinating protein and biomarker in neurology. Eur J Paediatr Neurol. 18(3):243-8 (2014). 
3. Wang X, et al. Alpha-Fetoprotein as a Predictive Marker for Patients with Hepatitis B-Related Acute-on-Chronic Liver Failure. Can J Gastroenterol Hepatol. 9;2018:1232785 (2018). 
4. Başbuğ D, et al. Is unexplained elevated maternal serum alpha-fetoprotein still important predictor for adverse pregnancy outcome? Ginekol Pol. 88(6):325-330 (2017).