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Recombinant Human ICAM-1/CD54 protein (His Tag)

ED50

/

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

3383

Accession

P05362

Cat No : Eg0570

Print datasheet

Synonyms

CD54,ICAM-1,BB2,ICAM 1,ICAM1

验证数据展示

  • Purity of Recombinant Human ICAM-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ICAM-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human ICAM-1 protein Gln28-Glu480 (Accession# P05362) with a His tag at the C-terminus.
Predicted Molecular Mass 53.6 kDa
SDS-PAGE 60-75 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. ICAM-1 can exist as membrane-bound form (mICAM-1) and soluble form (sICAM-1). The sICAM-1 arises from alternative splicing and proteolysis of mICAM-1 and appears to be associated with leukocyte activation to produce LFA-1.

References:

1. M L Dustin, et al. (1986) J Immunol. 137(1):245-54.
2. S D Marlin, et al. (1987) Cell. 51(5):813-9.
3. D E Staunton, et al. (1989) Cell. 56(5):849-53.
4. A R Berendt, et al. (1989) Nature. 341(6237):57-9.
5. Charlotte Lawson, et al. (2009) Pharmacol Rep. 61(1):22-32.