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Recombinant Mouse MIF protein (His Tag)

种属

Mouse

纯度

>85 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0574

Print datasheet

Synonyms

MIF, GIF

验证数据展示

  • Purity of Recombinant Mouse MIF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse MIF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >85 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Mouse MIF protein Pro2-Ala115 (Accession# P34884) with a His tag at the C-terminus.
基因ID 17319
蛋白编号 P34884
预测分子量 13.2 kDa
SDS-PAGE 16-20 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Macrophage migration inhibitory factor (MIF) is an upstream regulator of the innate and adaptive immune response that is widely expressed and that exhibits chemokine-like activities. MIF play a role in the pathogenesis of inflammatory diseases, such as atherosclerosis, rheumatoid arthritis, sepsis, asthma, and acute respiratory distress syndrome. MIF is expressed by the ischemic myocardium and that it may exhibit an overall cardioprotective role during I/R injury. MIF regulates inflammatory processes by recruiting immune cells, amplifying cytokine production, inhibiting apoptosis and counter-regulating the immunosuppressive action of glucocorticoids.

参考文献:

1. Calandra T. et al. (2003). Nat Rev Immunol. 3(10):791-800.
2. Koga K. et al.(2011). Antioxid Redox Signal. 14(7):1191-202.
3. Qi D. et al.(2009). J Clin Invest. 119(12):3807-16.
4. Stoppe C. et al.(2012). Mol Med.18:843-50.
5. Rajasekaran D. et al.(2014). 28(11):4961-71.
6. Bernhagen J. et al. (2007). 13(5):587-96.