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Recombinant Human rPTPσ (His tag)

ED50

/

Species

Human

Purity

>85 %, SDS-PAGE

GeneID

5802

Accession

Q13332

Cat No : Eg0576

Print datasheet

Synonyms

EC:3.1.3.48, PTPRS, PTPSIGMA, R PTP S, R PTP sigma, Receptor-type tyrosine-protein phosphatase S, Receptor-type tyrosine-protein phosphatase sigma, R-PTP-S, R-PTP-sigma, rPTPσ

验证数据展示

  • Purity of Recombinant Human rPTPσ was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human rPTPσ was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >85 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 

                                        		
                                    

                                    

                                        Source
                                        HEK293-derived Human rPTPσ protein Met825-Ile1000 (Accession# Q13332) with a His Tag at theC-terminus.
                                    

                                    

                                        Predicted Molecular Mass
                                        21 kDa
                                    

                                    

                                        SDS-PAGE
                                        20-30 kDa，reducing (R)  condition
                                    

                                    

                                        Formulation
                                        Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
                                    

                                    

                                        Reconstitution
                                        Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
                                    

                                    

                                        Storage
                                        
                                            
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

                                            
    
                                                
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
    • 
                                                
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
    • 
                                            

                                        		
                                    

                                    

                                        Shipping
                                        The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.
                                    

                                
                            

                        

                    

                

            

            

                

                    

                        

                            
Background

                            

                                

                                    Protein tyrosine phosphatase receptor type S (PTPRS, also known as R-PTP-S, PTPSIGMA, and R-PTP-sigma) is an integral membrane protein. It can promote oligomerization by binding to large heparan sulfate proteoglycan structures, and interact with NTRK1/3, PPFIA1/2/3 (PMID: 25385546; 8524829; 9624153). The non-glycosylated molecular weight of PTPRS is 214 kDa, and the extracellular domain can be cleaved near the cell membrane by an endoprotease to generate a glycated 140 kDa protein. Variations and mutations of PTPRS are associated with Alzheimer's disease and cholangiocarcinoma (PMID: 38766183; 35991009).
                                

                            

                            

                                
References:

                                
1. Coles CH, Mitakidis N. Nat Commun. 11;5:5209(2014).
2. Pulido R, Serra-Pagès C, Tang M. Proc Natl Acad Sci USA. 5;92(25):11686-90(1995).
3. Serra-Pagès C, Medley QG. J Biol Chem. 19;273(25):15611-20(1998). 
4. Poirier A, Picard C, Labonté A. bioRxiv [Preprint]. 12:2024.05.12.593733(2024).
5. Lertpanprom M, Silsirivanit A. Front Public Health. 1;10:835914(2022).