• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Mouse VCAM-1 (His Tag)

ED50

/

Species

Mouse

Purity

>90 %, SDS-PAGE

GeneID

22329

Accession

P29533

Cat No : Eg0639

Print datasheet

Synonyms

CD106, Vcam 1, Vcam1, VCAM-1

验证数据展示

  • Purity of Recombinant Mouse VCAM-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse VCAM-1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
/
Source HEK293-derived Mouse VCAM-1 protein Phe25-Glu698 (Accession# P29533) with a His Tag at the C-terminus.
Predicted Molecular Mass 75.2 kDa
SDS-PAGE 80-100 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Vascular cell adhesion molecule 1 (VCAM1) is a transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells. Soluble VCAM-1 (sVCAM-1) has been found in addition to membrane-bound VCAM-1.

References:

1. Elices MJ. et al. (1990) Cell. 60(4):577-84.
2. Pigott R. et al. (1992) Biochem Biophys Res Commun. 187(2):584-9.
3. Cybulsky MI. et al. (1993) Genomics. 18(2):387-91.
4. Tu Z. et al. (2001) J Immunol. 166(11):6839-46.