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Recombinant Human Osteopontin protein (His Tag)

种属

Human

纯度

>80 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0754

Print datasheet

Synonyms

SPP1, BNSP, Bone sialoprotein 1, BSPI, ETA 1

验证数据展示

  • Purity of Recombinant Human Osteopontin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Osteopontin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >80 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human Osteopontin protein Ile17-Asn300 (Accession# P10451-5) with a His tag at the C-terminus.
基因ID 6696
蛋白编号 P10451-5
预测分子量 32.9 kDa
SDS-PAGE 13 kDa, 17 kDa, 45-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Osteopontin (OPN), also known as SPP1, is a secreted glycophosphoprotein that belongs to the small integrin-binding ligand N-linked glycoprotein (SIBLING) family. Originally isolated from bone, OPN has been found in kidneys, vascular tissues, biological fluids, and various tumor tissues. OPN can interact with CD44 and integrins and regulate diverse biological processes. It has a multifaceted role in bone development and remodeling, and is also involved in the inflammatory and immune response, oncogenesis and cancer progression.

参考文献:

1. Rittling SR, Chambers AF. Br J Cancer. (2004) 17;90(10):1877-81. 
2. Rangaswami H, Bulbule A, Kundu GC. Trends Cell Biol. (2006) 16(2):79-87. 
3. Icer MA, Gezmen-Karadag M. Clin Biochem. (2018) 59:17-24. 
4. Jia Q, Ouyang Y, et al. Lung. (2024) 202(1):25-39. 
5. Tang Z, Xia Z, et al. Cytokine Growth Factor Rev. (2023) 74:86-99.