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Recombinant Human TFPI protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0855

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Synonyms

TFPI, EPI, Extrinsic pathway inhibitor, LACI, Lipoprotein-associated coagulation inhibitor

验证数据展示

  • Purity of Recombinant Human TFPI was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TFPI was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human TFPI protein Asp29-Lys282 (Accession# P10646-1) with a His tag at the C-terminus.
基因ID 7035
蛋白编号 P10646-1
预测分子量 30.8 kDa
SDS-PAGE 38-50 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Tissue factor pathway inhibitor (TFPI) is a critical anticoagulant protein present in endothelium and platelets. TFPI is produced as two major isoforms in humans, TFPIa and TFPIb that result from alternative splicing. The TFPIb isoform localizes to the endothelium surface where it is a potent inhibitor of tissue factor-factor VIIa complexes that initiate blood coagulation. The TFPIa isoform is present in platelets. TFPI is a Kunitz-type serine protease inhibitor that exerts anticoagulant activity by blocking early procoagulant stimulia. TFPI can enhance anti-thrombotic treatment in sepsis, inflammatory diseases, and cardiovascular diseases. In plasma, 80% of TFPI is carboxy-terminal truncated and circulates bound primarily to low-density lipoproteins (LDL), with the levels of the latter having an impact on plasma TFPI levels. The other 20% is unbound free-form TFPI characterised by the K3 domain.

参考文献:

1. Alan E Mast. et al. (2016) Arterioscler Thromb Vasc Biol. 36(1):9-14.
2. Jeremy P Wood. et al. (2014) Blood. 123(19):2934-43.
3. N L Sanders. et al. (1985) Blood. 66(1):204-12.
4. Lwaleed BA. et al. (2006) J Pathol. 208(3): 327-39.