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Recombinant Human Fas/CD95 protein (His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 1-5 ng/mL

Cat no : Eg0877

Print datasheet

Synonyms

Fas/TNFRSF6, FAS, ALPS1A, APO-1, Apo-1 antigen

验证数据展示

  • Purity of Recombinant Human Fas was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Fas was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.

    Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human Fas (His tag) at 2 μg/mL (100 μL/well) can bind Human Fas Ligand (hFc tag) with a linear range of 1-5 ng/mL.
来源 HEK293-derived Human Fas protein Gln26-Asn173 (Accession# P25445-1) with His tag at the C-terminus.
基因ID 355
蛋白编号 P25445-1
预测分子量 17.4 kDa
SDS-PAGE 20-36 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Fas, also known as TNFRSF6, CD95, and APO-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis plays a role in the maintenance of cell homeostasis and in the deletion of useless or autoreactive T cells. Alterations in the CD95/CD95L pathway have been involved in several disease conditions, including autoimmune diseases, chronic inflammation and cancer.

参考文献:

1.N Itoh. et al. (1991). Cell. 66(2):233-43.
2.M E Peter. et al. (2003). Cell Death Differ. 10(1):26-35.
3.M E Peter. et al. (2015). Cell Death Differ. 22(4):549-59.
4.Vesna Risso. et al. (2022). Cell Death Dis. 13(3):248.