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Recombinant Rat Leptin (His Tag)

ED50

/

Species

Rat

Purity

>90 %, SDS-PAGE

GeneID

25608

Accession

P50596

Cat No : Eg0892

Print datasheet

Synonyms

Lep, leptin, OB

验证数据展示

  • Purity of Recombinant Rat Leptin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Rat Leptin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
/
Source HEK293-derived Rat Leptin protein Val22-Cys167 (Accession# P50596) with a His Tag at the C-terminus.
Predicted Molecular Mass 17 kDa
SDS-PAGE 10-20 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Leptin is a 16 kDa adipocyte-derived hormone, and it has attracted interest in the field of obesity research due to its role in the regulation of energy balance. Leptin, secreted from adipose tissues in proportion to the fat store, acts in the hypothalamus to regulate feeding behavior. leptin has also been shown to play an essential role in tumorigenesis, tumor progression, and metastasis, due to its oncogenic, mitogenic, pro-inflammatory, and pro-angiogenic actions. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. Leptin has also been linked to type 2 diabetes mellitus development.

References:

1. Arnold M. et al. (2016) Cancer Epidemiol. 41:8-15.
2. Zhang Y. et al. (1994) Nature. 37:425-32.
3. de Luis DA. et al. (2009) Minerva Med. 229-36.
4. Jiang N. et al.(2014) Drug Des Devel Ther. 8:2295-302.
5. Vansaun MN. et al.(2013) Clin Cancer Res. 19:1926-1932.