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Recombinant Human HIF-1 alpha/HIF1A protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

N-His

生物活性

未测试

Cat no : Eg0939

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Synonyms

HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78

验证数据展示

  • Purity of Recombinant Human HIF-1 alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) stained using Coomassie blue.

    Purity of Recombinant Human HIF-1 alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human HIF-1 alpha protein Arg575-Asn826 (Accession# Q16665-1) with a His tag at the N-terminus.
基因ID 3091
蛋白编号 Q16665-1
预测分子量 28.3 kDa
SDS-PAGE 34-60 kDa, reducing (R) condition
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

HIF1a, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1 alpha (HIF1A), and an oxygen-insensitive subunit, HIF1 beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Under normal oxygen conditions, HIF1a is continuously produced and destroyed, in a process involving hydroxylation, interaction with von Hippel-Lindau (VHL) protein, polyubiquitylation and subsequent proteasomal degradation. Under hypoxic conditions, hydroxylation is impaired and HIF1a is stabilized. HIF1a localizes in cytoplasm in normoxia, but it can translocate into nuclear in response to hypoxia.

参考文献:

1.J E Albina, et al. (2001) Am J Physiol Cell Physiol. 281 (6):C1971-7.
2.Wang GL, et al. (1995) Proc Natl Acad Sci U S A.92 (12):5510-4.
3.Kubis HP, et al. (2005) Biochim Biophys Acta. 1745 (2):187-95.