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Recombinant Human Thrombomodulin (His Tag)

ED50

/

Species

Human

Purity

>85 %, SDS-PAGE

GeneID

7056

Accession

P07204

Cat No : Eg0944

Print datasheet

Synonyms

THBD

验证数据展示

Technical Specifications

Purity >85 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
/
Source HEK293-derived Human Thrombomodulin protein Ala19-Ser515 (Accession# P07204) with a His Tag at the C-terminus.
Predicted Molecular Mass 52.9 kDa
SDS-PAGE 70-100 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Thrombomodulin, also known as CD141, is an endothelial cell surface glycoprotein that forms a 1:1 complex with the coagulation factor thrombin and plays an important role as a natural anticoagulant. Thrombomodulin serves to convert thrombin from a procoagulant protein into the activator for protein C. Once converted to activated protein C (APC), this protein serves as a major anticoagulant in blood. Thrombomodulin is also located in other cells (keratinocytes, osteoblasts, macrophages,...) where it might be involved in cell differentiation or in inflammation. Mutations in the gene of thrombomodulin are a cause of thromboembolic disease, also known as inherited thrombophilia.

References:

1. N L Esmon, et al. (1987) Semin Thromb Hemost. 13(4):454-63.
2. A K Ohlin, et al. (1997) Thromb Haemost. 78(1):396-400.
3. M C Boffa, et al. (1998) Lupus.7 Suppl 2:S120-5.
4. Georgia Anastasiou, et al. (2012) Blood Coagul Fibrinolysis. 23(1):1-10.