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Recombinant Human ASGR1/ASGPR1 protein (His Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

432

Accession

P07306-1

Cat No : Eg0946

Print datasheet

Synonyms

ASGR1,asialoglycoprotein receptor 1,HL-1,ASGP R 1,

验证数据展示

  • Purity of Recombinant Human ASGR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human ASGR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human ASGR1 protein Gln62-Leu291 (Accession# P07306-1) with a His tag at the N-terminus.
Predicted Molecular Mass 27.2 kDa
SDS-PAGE 32-38 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. ASGPR plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues.

References:

1.Meier, M. et al. (2000).  J. Mol. Biol. 300:857.
2.Rigopoulou EI. et al. (2012). Autoimmun Rev. 12(2):260-9. 
3.Weigel, P.H. and J.H.N. Yik (2002). Biochim. Biophys. Acta 1572:341.
4.Meier, M. et al. (2000).  J. Mol. Biol. 300:857.