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Recombinant Mouse Apolipoprotein E/APOE3 protein (His Tag)

ED50

/

Species

Mouse

Purity

>90 %, SDS-PAGE

GeneID

11816

Accession

P08226

Cat No : Eg1093

Print datasheet

Synonyms

Apoe,AI255918,apolipoprotein E

验证数据展示

  • Purity of Recombinant Mouse Apolipoprotein E was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse Apolipoprotein E was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Mouse Apolipoprotein E protein Glu19-Gln311 (Accession# P08226) with a His tag at the C-terminus.
Predicted Molecular Mass 34.8 kDa
SDS-PAGE 30-32 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Apolipoprotein E (APOE) was first identified in the 1970s as one of the protein components of plasma very-low-density lipoprotein (VLDL) and found to play a critical role in plasma cholesterol metabolism. APOE is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serves as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

References:

1. Montine K S, et al. (1998). J Lipid Res. 39,12: 2443-51.
2. Chen Y, et al. (2021). Neuron. 109(2):205-221.
3. Koutsodendris N, et al. (2022). Annu Rev Pathol. 17:73-99.