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Recombinant Human E-cadherin protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg1112

Print datasheet

Synonyms

E-cadherin, Arc 1, Cadherin 1, Cadherin-1, CAM 120/80

验证数据展示

  • Purity of Recombinant Human E-cadherin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human E-cadherin was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human E-cadherin protein Asp155-Ala709 (Accession# P12830-1) with a His tag at the C-terminus.
基因ID 999
蛋白编号 P12830-1
预测分子量 61.6 kDa
SDS-PAGE 65-80 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness. E-cadherin is sensitive to trypsin digestion in the absence of Ca2+.

参考文献:

1.Braga V. et al. (2016) Curr Opin Cell Biol. 42:138-21.
2.Ishiyama N. et al. (2017) Cold Spring Harb Perspect Biol. 9(5):a028738.
3.Dongre A. et al. (2019) Nat Rev Mol Cell Biol. 20(2):69-84.
4.Yang, S. et al. (2013) Biomed res int. 2013:608728.
5.Harrison OJ. et al. (2010) Nat Struct Mol Biol. 17(3):348-357.
6.Biswas KH. et al. (2017) Exp Cell Res.358(1):14-19.
7.Buckley CD. et al. (2014) Science. 346(6209):1254211.