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Recombinant Human PD-L1/CD274 (His Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

29126

Accession

Q9NZQ7

Cat No : Eg1114

Print datasheet

Synonyms

B7 H1, B7-H1, CD274, hPD-L1, PD L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, PDL1, PD-L1

验证数据展示

  • Purity of Recombinant Human PD-L1/CD274 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PD-L1/CD274 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
/
Source HEK293-derived Human PD-L1/CD274 protein Phe19-Arg238 (Accession# Q9NZQ7) with a His Tag at the C-terminus.
Predicted Molecular Mass 26 kDa
SDS-PAGE 35-45 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

PD-L1 (programmed cell death ligand 1, also known as CD274 or B7-H1) is a 290 aa type I transmembrane protein. PD-L1 is expressed constitutively on T cells, B cells, DCs, macrophages, mesenchymal stem cells and cultured bone marrow-derived mast cells. In addition, PD-L1 is also expressed on many nonhematopoietic cell types, including vascular endothelial cells, epithelial cells, muscle cells, hepatocytes, pancreatic islet cells, astrocytes in the brain, placental syncytiotrophoblasts, and cells in cornea, iris-ciliary body and retina of eye. PD-L1 is frequently upregulated in a wide variety of solid tumors, including melanoma, ovarian, lung, glioblastoma, breast, and pancreatic cancers. PD-L1 and PD-L2 are two ligands of PD-1. Engagement of PD-1 by PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function. It is critical for the regulation of T cell function during tolerance, autoimmunity and infection. Besides the membrane-bound form, PD-L1 can also exist as a soluble form (sPD-L1) generated either by proteolytic cleavage of membrane-bound form or by translation of alternative spliced mRNA.

References:

1. Arlene H Sharpe, et al. (2007) Nat Immunol. 8(3):239-45.
2. Mary E Keir, et al. (2008) Annu Rev Immunol. 26:677-704.
3. James L Riley. (2009) Immunol Rev. 229(1):114-25.
4. Masahiro Takeuchi, et al. (2018) Immunol Lett. 196:155-160.