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Recombinant Human Complement factor D protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg1189

Print datasheet

Synonyms

CFD, ADIPSIN, C3 convertase activator, complement factor D (adipsin), DF

验证数据展示

  • Purity of Recombinant Human Complement factor D was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Complement factor D was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human Complement factor D protein Ile26-Ala253 (Accession# P00746) with a His tag at the C-terminus.
基因ID 1675
蛋白编号 P00746
预测分子量 25.5 kDa
SDS-PAGE 23-26 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Complement Factor D, also known as CFD or adipsin, is a serine protease. Unlike most proteins of the complement system, which are synthesized by the liver and immune cells, factor D is predominantly produced by and secreted into the bloodstream by adipocytes. However, it is also synthesized by macrophages and monocytes, as well as by brain astrocytes to a lesser extent. factor D plays a role in adipose tissue. Adipocytes are energy reservoirs that play an important role in energy balance. Not only are they responsible for lipolysis but they are also involved in glucose uptake and triglyceride synthesis.

参考文献:

1. Jonathan Barratt. et al. (2021). Front Immunol. 12:712572.
2. White RT. et al.(1992). J Biol Chem. 267(13):9210–9213.
3. Choy LN. et al. (1992). J Biol Chem. 267(18):12736–12741.
4. Song N-J. et al. (2016). PloS One.11(9):e0162228.