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Recombinant Human HSP47/SERPINH1 protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg1201

Print datasheet

Synonyms

HSP47, SERPINH1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3

验证数据展示

  • Purity of Recombinant Human HSP47 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human HSP47 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human HSP47 protein Ala19-Leu418 (Accession# P50454) with a His tag at the C-terminus.
基因ID 871
蛋白编号 P50454
预测分子量 45.3 kDa
SDS-PAGE 48-60 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

HSP47/SERPINH1 is also named as Serpin H1, CBP1, CBP2, 47 kDa heat shock protein, Arsenic-transactivated protein 3 (AsTP3) and Collagen-binding protein (Colligin). It belongs to the serpin family. The Serpin Family H Member 1 (SERPINH1) gene encodes a protein called HSP47, which is required for the correct folding and secretion of collagen. SERPINH1 has been implicated in several collagen-related diseases, such as, brittle bone disease, keloid scars, and lung fibrosis. SERPINH1 also correlates with the grade of glioma, and regulates proliferation, migration and invasion of glioma cell lines. SERPINH1 regulates the Wnt/β-catenin signaling pathway and modulates gastric cancer (GC) progression. SERPINH1 is aberrantly expressed in cervical cancer, breast cancer, glioblastoma and colorectal cancer. SERPINH1 promotes invasion and metastasis of breast cancer cells by regulating the expression of several extracellular matrix (ECM) proteins.

参考文献:

1. Duarte BDP, Bonatto D. (2018) J Cancer Res Clin Oncol. 144(12):2319-2328. 
2. Tian S, et al. (2020) Aging (Albany NY). 12(4):3574-3593. 
3. Zhao D, et al. (2014) J Neurooncol. 118(1):39-47. 
4. Zhu J, et al.  (2015) Cancer Res. 75(8):1580-91.