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Recombinant Human Complement factor B protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg1224

Print datasheet

Synonyms

CFB, Complement factor B, BF, BFD, C3/C5 convertase

验证数据展示

  • Purity of Recombinant Human Complement factor B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human Complement factor B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human Complement factor B protein Thr26-Leu764 (Accession# P00751-1) with a His tag at the C-terminus.
基因ID 629
蛋白编号 P00751-1
预测分子量 87.1 kDa
SDS-PAGE 85-100 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Complement Factor B (CFB) is a vital component of the alternative pathway of the complement system, playing a central role in immune responses and inflammation. CFB is synthesized mainly by hepatocytes and macrophages and is involved in the defense against cellular damage and inflammation. It binds to C3b and is cleaved by Factor D to form the active Bb fragment, which then combines with C3b to generate the C3 or C5 convertase, initiating an inflammatory response, cytolysis, phagocytosis, and B cell activation. CFB is functionally similar to the classical pathway component C2, as both form the catalytic subunit of C3 convertases after activation and must be bound to be active.

参考文献:

1. Wenjun Wang. et al. (2021). Dev Comp Immunol. 118:103992.
2. Eva Torreira. et al. (2009). J Immunol. 183(11):7347-7351.
3. Lin Zou. et al. (2016). J Immunol. 196(6):2788-2798.