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Recombinant Human CD63 protein (rFc Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

967

Accession

P08962

Cat No : Eg2064

Print datasheet

Synonyms

CD63,CD 63,CD63 antigen,Limp1,Lysosomal-associated

验证数据展示

  • Purity of Recombinant Human CD63 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD63 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human CD63 protein Ala103-Val203 (Accession# P08962) with a rabbit IgG Fc tag at the C-terminus.
Predicted Molecular Mass 37.6 kDa
SDS-PAGE 42-58 kDa, reducing (R) condition
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it as a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

References:

1. Ki-Kyung Jung, et al. (2006) EMBO J. Sep 6;25(17):3934-42.
2. Guillaume van Niel, et al. (2011) Dev Cell. Oct 18;21(4):708-21.
3. Maaike S Pols, Judith Klumperman. (2009) Exp Cell Res. May 15;315(9):1584-92.
4. D O Azorsa, et al. (1991) Blood. Jul 15;78(2):280-4.
5. M Nishibori, et al. (1993) J Clin Invest. Apr;91(4):1775-82.Maaike S Pols 1, Judith Klumperman