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Recombinant Mouse TNF-alpha protein (Myc Tag, His Tag)

种属

Mouse

纯度

>90 %, SDS-PAGE

标签

Myc Tag, His Tag

生物活性

未测试

Cat no : Eg31366

Print datasheet

Synonyms

Tnf, Tnfa, DIF, TNF a, TNF alpha

验证数据展示

  • Purity of Recombinant Mouse TNF-alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Mouse TNF-alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Mouse TNF-alpha protein Leu80-Leu235 (Accession# P06804) with a Myc tag and a His tag at the C-terminus.
基因ID 21926
蛋白编号 P06804
预测分子量 22.3 kDa
SDS-PAGE 22-26 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

TNF, also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Mouse and human TNF-alpha share 79% amino acid sequence identity. Unlike human TNF-alpha, the mouse form is glycosylated. In mouse deficiency of this gene is associated with defects in response to bacterial infection, with defects in forming organized follicular dendritic cell networks and germinal centers, and with a lack of primary B cell follicles.

参考文献:

1. Agbanoma G. et al. (2012) J Immunol. 188: 1307-17.
2. Kriegler M. et al. (1988) Cell. 53: 45-53.
3. Theiss AL. et al. (2005) J Biol Chem. 280: 36099-109.
4. Swardfager W. et al. (2010) Biol Psychiatry. 68:930-41.
5. Locksley RM.et al. (2001) Cell. 104(4):487-501.
6. provided by RefSeq, Jun 2013