Human MCP-1/CCL2 ELISA Kit
Sensitivity
0.5 pg/mL
Range
7.8-500 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00425 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human MCP-1/CCL2 ELISA kit is to be used to detect and quantify protein levels of endogenous human MCP-1/CCL2. The assay recognizes human MCP-1/CCL2. An antibody specific for human MCP-1/CCL2 has been pre-coated onto the microwells. The human MCP-1/CCL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human MCP-1/CCL2 is added to detect the captured human MCP-1/CCL2 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human MCP-1/CCL2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Urine |
| Assay type | Sandwich |
| Sensitivity | 0.5 pg/mL |
| Range | 7.8-500 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6347 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 95% | 89%-102% |
| Cell culture supernatant | 102% | 98%-107% |
| Urine | 92% | 89%-98% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 272.4 | 12.5 | 4.6 |
| 2 | 20 | 61.4 | 0.6 | 1.0 |
| 3 | 20 | 14.9 | 0.3 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 274.0 | 17.6 | 6.4 |
| 2 | 24 | 61.8 | 2.6 | 4.3 |
| 3 | 24 | 15.7 | 0.4 | 2.6 |
Background Information
Monocyte chemotactic protein 1 (MCP1; also known as CCL2), is a chemokine that can be expressed in monocytes, macrophages, and endothelial cells, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. Research has shown that the expression of MCP1 increases in the serum of patients with acute myocarditis. MCP1 is up-regulated in many types of CNS injury, including ischemia, hemorrhage, trauma, infection, hypoxia, and peripheral nerve axotomy. MCP1 has also been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CCL2,MCP1,MCP-1,C C motif chemokine 2,C-C motif ch |