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ABCA3 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00121-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00121-3, Human ABCA3 Recombinant Matched Antibody Pair - PBS only. 83018-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24749. 83018-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

    Sandwich ELISA standard curve of MP00121-3

    Sandwich ELISA standard curve of MP00121-3, Human ABCA3 Recombinant Matched Antibody Pair - PBS only. 83018-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag24749. 83018-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Hela and SW480 cell lysates were measured. The human ABCA3 concentration of detected samples was determined to be 2.8 ng/mL (based on a 2.1 mg/mL extract load) in Hela cell lysate and 1.9 ng/mL (based on a 2.3 mg/mL extract load) in SW480 cell lysate.

    Sample test of MP00121-3

    Hela and SW480 cell lysates were measured. The human ABCA3 concentration of detected samples was determined to be 2.8 ng/mL (based on a 2.1 mg/mL extract load) in Hela cell lysate and 1.9 ng/mL (based on a 2.3 mg/mL extract load) in SW480 cell lysate.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00121-3 targets ABCA3 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: ABCA3 Recombinant antibody, PBS Only (Capture) 83018-5-PBS (230194A9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: ABCA3 Recombinant antibody, PBS Only (Detector) 83018-4-PBS (230194C6). 100 μg. Concentration 1 mgl/ml.

Alternative ABCA3 matched antibody pairs: MP00121-1, MP00121-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83018-5-PBS
Tested Reactivity human
Immunogen ABCA3 fusion protein Ag24749
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATP-binding cassette, sub-family A (ABC1), member 3
Synonyms ABC-C transporter, ABCA 3, ABC3, ABC C transporter, 230194A9
GenBank accession numberBC140895
Gene symbol ABCA3
UniprotQ99758
Gene ID (NCBI) 21
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83018-4-PBS
Tested Reactivity human
Immunogen ABCA3 fusion protein Ag24749
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATP-binding cassette, sub-family A (ABC1), member 3
Synonyms ABC-C transporter, ABCA 3, ABC3, ABC C transporter, 230194C6
GenBank accession numberBC140895
Gene symbol ABCA3
UniprotQ99758
Gene ID (NCBI) 21
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C