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AGXT Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.195-12.5 ng/mL

Cat No : MP00420-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00420-4, Human AGXT Recombinant Matched Antibody Pair - PBS only. 83385-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag18062. 83385-3-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

    Sandwich ELISA standard curve of MP00420-4

    Sandwich ELISA standard curve of MP00420-4, Human AGXT Recombinant Matched Antibody Pair - PBS only. 83385-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag18062. 83385-3-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL

  • HepG2, L02 and NIC-H1299 cell lysates were measured. The human AGXT concentration of detected samples was determined to be 95.31 ng/mL (based on a 1.2 mg/mL extract load) in HepG2 cell lysate, 14.94 ng/mL (based on a 2.6 mg/mL extract load) in L02 cell lysate and 11.79 ng/mL (based on a 1.2 mg/mL extract load) in NIC-H1299 cell lysate.

    Sample test of MP00420-4

    HepG2, L02 and NIC-H1299 cell lysates were measured. The human AGXT concentration of detected samples was determined to be 95.31 ng/mL (based on a 1.2 mg/mL extract load) in HepG2 cell lysate, 14.94 ng/mL (based on a 2.6 mg/mL extract load) in L02 cell lysate and 11.79 ng/mL (based on a 1.2 mg/mL extract load) in NIC-H1299 cell lysate.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00420-4 targets AGXT in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.195-12.5 ng/mL (Sandwich ELISA);

Capture antibody: AGXT Recombinant antibody, PBS Only (Capture) 83385-5-PBS (240305F9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: AGXT Recombinant antibody, PBS Only (Detector) 83385-3-PBS (240305E11). 100 μg. Concentration 1 mgl/ml.

Alternative AGXT matched antibody pairs: MP00420-1, MP00420-2, MP00420-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83385-5-PBS
Tested Reactivity human
Immunogen AGXT fusion protein Ag18062
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alanine-glyoxylate aminotransferase
Synonyms EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT
GenBank accession numberBC132819
Gene symbol AGXT
UniprotP21549
Gene ID (NCBI) 189
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83385-3-PBS
Tested Reactivity human
Immunogen AGXT fusion protein Ag18062
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alanine-glyoxylate aminotransferase
Synonyms EC:2.6.1.44, Alanine--glyoxylate aminotransferase, AGXT1, AGT1, AGT
GenBank accession numberBC132819
Gene symbol AGXT
UniprotP21549
Gene ID (NCBI) 189
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C