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HSPA2 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human, mouse

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-80 ng/mL

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP00465-1

Print datasheet

Synonyms

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00465-1, Human HSPA2 Recombinant Matched Antibody Pair - PBS only. 83466-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3539. 83466-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

    Sandwich ELISA standard curve of MP00465-1

    Sandwich ELISA standard curve of MP00465-1, Human HSPA2 Recombinant Matched Antibody Pair - PBS only. 83466-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3539. 83466-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • The mean HSPA2 concentration was determined to be 34.57 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

    Sample test of MP00465-1

    The mean HSPA2 concentration was determined to be 34.57 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

  • Cytometric bead array standard curve of MP00465-1, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-3-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

    Cytometric bead array standard curve of MP00465-1

    Cytometric bead array standard curve of MP00465-1, HSPA2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83466-1-PBS. Detection antibody: 83466-3-PBS. Standard: Ag3539. Range: 0.625-80 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00465-1 targets HSPA2 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-80 ng/mL (Cytometric Bead Array); 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: HSPA2 Recombinant antibody, PBS Only (Capture) 83466-1-PBS (240421A2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: HSPA2 Recombinant antibody, PBS Only (Capture/Detector) 83466-3-PBS (240421A4). 100 μg. Concentration 1 mgl/ml.

Alternative HSPA2 matched antibody pairs: MP00465-2, MP00465-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83466-1-PBS
Tested Reactivity human, mouse
Immunogen HSPA2 fusion protein Ag3539
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock 70kDa protein 2
Synonyms HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2
GenBank accession numberBC036107
Gene symbol HSPA2
UniprotP54652
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83466-3-PBS
Tested Reactivity human
Immunogen HSPA2 fusion protein Ag3539
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock 70kDa protein 2
Synonyms HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A4
GenBank accession numberBC036107
Gene symbol HSPA2
UniprotP54652
Gene ID (NCBI) 3306
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C