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RAE1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP00683-4

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00683-4, Human RAE1 Recombinant Matched Antibody Pair - PBS only. 83712-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12102. 83712-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

    Sandwich ELISA standard curve of MP00683-4

    Sandwich ELISA standard curve of MP00683-4, Human RAE1 Recombinant Matched Antibody Pair - PBS only. 83712-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12102. 83712-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Colo320 and MCF-7 cell lysates were measured. The human RAE1 concentration of detected samples was determined to be 123.92 ng/mL (based on a 2.00 mg/mL extract load) in Colo320 cell lysate and 93.86 ng/mL (based on a 1.20 mg/mL extract load) in MCF-7 cell lysate.

    Sample test of MP00683-4

    Colo320 and MCF-7 cell lysates were measured. The human RAE1 concentration of detected samples was determined to be 123.92 ng/mL (based on a 2.00 mg/mL extract load) in Colo320 cell lysate and 93.86 ng/mL (based on a 1.20 mg/mL extract load) in MCF-7 cell lysate.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00683-4 targets RAE1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: RAE1 Recombinant antibody, PBS Only (Capture) 83712-5-PBS (240861G1). 100 μg. Concentration 1 mgl/ml.

Detection antibody: RAE1 Recombinant antibody, PBS Only (Capture/Detector) 83712-3-PBS (240861H8). 100 μg. Concentration 1 mgl/ml.

Alternative RAE1 matched antibody pairs: MP00683-1, MP00683-2, MP00683-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83712-5-PBS
Tested Reactivity human
Immunogen RAE1 fusion protein Ag12102
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAE1 RNA export 1 homolog (S. pombe)
Synonyms mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14
GenBank accession numberBC103754
Gene symbol RAE1
UniprotP78406
Gene ID (NCBI) 8480
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83712-3-PBS
Tested Reactivity human
Immunogen RAE1 fusion protein Ag12102
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name RAE1 RNA export 1 homolog (S. pombe)
Synonyms mRNA-associated protein mrnp 41, mRNA export factor RAE1, mRNA export factor, Mnrp41, MIG14
GenBank accession numberBC103754
Gene symbol RAE1
UniprotP78406
Gene ID (NCBI) 8480
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C