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ATP2B1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.625-40 ng/mL

Cat No : MP00808-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00808-3, Human ATP2B1 Recombinant Matched Antibody Pair - PBS only. 83827-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32423. 83827-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

    Sandwich ELISA standard curve of MP00808-3

    Sandwich ELISA standard curve of MP00808-3, Human ATP2B1 Recombinant Matched Antibody Pair - PBS only. 83827-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32423. 83827-5-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • The mean ATP2B1 concentration was determined to be 29.13 ng/mL in HUVEC cell extract based on a 0.91 mg/mL extract load.

    Sample test of MP00808-3

    The mean ATP2B1 concentration was determined to be 29.13 ng/mL in HUVEC cell extract based on a 0.91 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00808-3 targets ATP2B1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.625-40 ng/mL (Sandwich ELISA);

Capture antibody: ATP2B1 Recombinant antibody, PBS Only (Capture/Detector) 83827-2-PBS (240848D2). 100 μg. Concentration 1 mgl/ml.

Detection antibody: ATP2B1 Recombinant antibody, PBS Only (Detector) 83827-5-PBS (240848A11). 100 μg. Concentration 1 mgl/ml.

Alternative ATP2B1 matched antibody pairs: MP00808-1, MP00808-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83827-2-PBS
Tested Reactivity human
Immunogen ATP2B1 fusion protein Ag32423
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Ca++ transporting, plasma membrane 1
Synonyms PMCA1kb, PMCA1, 240848D2
GenBank accession numberNM_001001323.1
Gene symbol ATP2B1
UniprotP20020
Gene ID (NCBI) 490
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83827-5-PBS
Tested Reactivity human
Immunogen ATP2B1 fusion protein Ag32423
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ATPase, Ca++ transporting, plasma membrane 1
Synonyms PMCA1kb, PMCA1, 240848A11
GenBank accession numberNM_001001323.1
Gene symbol ATP2B1
UniprotP20020
Gene ID (NCBI) 490
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C