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Kininogen-1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.781-100 ng/mL

Sandwich ELISA: 39.1-2500 pg/mL

Cat No : MP01081-2

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Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01081-2, Human Kininogen-1 Recombinant Matched Antibody Pair - PBS only. 84175-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1162. 84175-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500pg/mL

    Sandwich ELISA standard curve of MP01081-2

    Sandwich ELISA standard curve of MP01081-2, Human Kininogen-1 Recombinant Matched Antibody Pair - PBS only. 84175-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1162. 84175-2-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500pg/mL

  • Serum of six individual healthy human donors was measured. The human Kininogen-1 concentration of detected samples was determined to be 21,735.55 ng/mL with a range of 2,325.73 - 45,247.60 ng/mL.

    Sample test of MP01081-2

    Serum of six individual healthy human donors was measured. The human Kininogen-1 concentration of detected samples was determined to be 21,735.55 ng/mL with a range of 2,325.73 - 45,247.60 ng/mL.

  • Saliva of five individual healthy human donors was measured. The human Kininogen-1 concentration of detected samples was determined to be 1.55 ng/mL with a range of 0.70 - 2.43 ng/mL.

    Sample test of MP01081-2

    Saliva of five individual healthy human donors was measured. The human Kininogen-1 concentration of detected samples was determined to be 1.55 ng/mL with a range of 0.70 - 2.43 ng/mL.

  • HepG2 (human hepatocellular carcinoma cells) was cultured in DMEM supplemented with 10% fetal bonive serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Kininogen-1, and measured 458.7 pg/mL.

    Sample test of MP01081-2

    HepG2 (human hepatocellular carcinoma cells) was cultured in DMEM supplemented with 10% fetal bonive serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human Kininogen-1, and measured 458.7 pg/mL.

  • Cytometric bead array standard curve of MP01081-2, Kininogen-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84175-4-PBS. Detection antibody: 84175-2-PBS. Standard: Eg1162. Range: 0.781-100 ng/mL.

    Cytometric bead array standard curve of MP01081-2

    Cytometric bead array standard curve of MP01081-2, Kininogen-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84175-4-PBS. Detection antibody: 84175-2-PBS. Standard: Eg1162. Range: 0.781-100 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01081-2 targets Kininogen-1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.781-100 ng/mL (Cytometric Bead Array); 39.1-2500 pg/mL (Sandwich ELISA);

Capture antibody: Kininogen-1 Recombinant antibody, PBS Only (Capture) 84175-4-PBS (241397G9). 100 μg. Concentration 1 mgl/ml.

Detection antibody: Kininogen-1 Recombinant antibody, PBS Only (Detector) 84175-2-PBS (241397C5). 100 μg. Concentration 1 mgl/ml.

Alternative Kininogen-1 matched antibody pairs: MP01081-1, MP01081-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84175-4-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kininogen 1
Synonyms KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK
GenBank accession numberNM_001102416.3
Gene symbol Kininogen 1
UniprotP01042-1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 84175-2-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name kininogen 1
Synonyms KNG1, HMWK, Fitzgerald factor, Bradykinin, BDK
GenBank accession numberNM_001102416.3
Gene symbol Kininogen 1
UniprotP01042-1
Gene ID (NCBI) 3827
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C