• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TNFSF8 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-80 ng/mL

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP01420-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01420-2, Human TNFSF8 Recombinant Matched Antibody Pair - PBS only. 84586-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2118. 84586-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

    Sandwich ELISA standard curve of MP01420-2

    Sandwich ELISA standard curve of MP01420-2, Human TNFSF8 Recombinant Matched Antibody Pair - PBS only. 84586-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2118. 84586-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Serum of nine individual healthy human donors was measured. The human TNFSF8 concentration of detected samples was determined to be 1.10 ng/mL with a range of 0.94 - 1.25 ng/mL.

    Sample test of MP01420-2

    Serum of nine individual healthy human donors was measured. The human TNFSF8 concentration of detected samples was determined to be 1.10 ng/mL with a range of 0.94 - 1.25 ng/mL.

  • The mean TNFSF8 concentration was determined to be 16.00 ng/mL in Ramos cell extract based on a 1.4 mg/mL extract load.

    Sample test of MP01420-2

    The mean TNFSF8 concentration was determined to be 16.00 ng/mL in Ramos cell extract based on a 1.4 mg/mL extract load.

  • Cytometric bead array standard curve of MP01420-2, TNFSF8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84586-3-PBS. Detection antibody: 84586-2-PBS. Standard: Eg2118. Range: 0.625-80 ng/mL.

    Cytometric bead array standard curve of MP01420-2

    Cytometric bead array standard curve of MP01420-2, TNFSF8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84586-3-PBS. Detection antibody: 84586-2-PBS. Standard: Eg2118. Range: 0.625-80 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01420-2 targets TNFSF8 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-80 ng/mL (Cytometric Bead Array); 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: TNFSF8 Recombinant antibody, PBS Only (Capture) 84586-3-PBS (241831D12). 100 μg. Concentration 1 mgl/ml.

Detection antibody: TNFSF8 Recombinant antibody, PBS Only (Detector) 84586-2-PBS (241831D9). 100 μg. Concentration 1 mgl/ml.

Alternative TNFSF8 matched antibody pairs: MP01420-1, MP50555-1, MP50555-2, MP50555-3

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84586-3-PBS
Tested Reactivity human
Immunogen FusionProtein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 8
Synonyms TNFSF 8, CD30 L, 241831D12, CD30LG, CD30-L
GenBank accession numberBC093630
Gene symbol TNFSF8
UniprotP32971
Gene ID (NCBI) 944
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 84586-2-PBS
Tested Reactivity human
Immunogen FusionProtein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 8
Synonyms TNFSF 8, CD30 L, 241831D9, CD30LG, CD30-L
GenBank accession numberBC093630
Gene symbol TNFSF8
UniprotP32971
Gene ID (NCBI) 944
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C