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SOX17 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.156-20 ng/mL

Sandwich ELISA: 0.625-40 ng/mL

Cat No : MP01724-2

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01724-2, Human SOX17 Recombinant Matched Antibody Pair - PBS only. 84936-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag19157. 84936-4-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

    Sandwich ELISA standard curve of MP01724-2

    Sandwich ELISA standard curve of MP01724-2, Human SOX17 Recombinant Matched Antibody Pair - PBS only. 84936-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag19157. 84936-4-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  • The mean SOX17 concentration was determined to be 13.82 ng/mL in SKOV-3 cell extract based on a 1.20 mg/mL extract load and 16.82 ng/mL in A2780 cell extract based on a 1.20 mg/mL extract load.

    Sample test of MP01724-2

    The mean SOX17 concentration was determined to be 13.82 ng/mL in SKOV-3 cell extract based on a 1.20 mg/mL extract load and 16.82 ng/mL in A2780 cell extract based on a 1.20 mg/mL extract load.

  • Cytometric bead array standard curve of MP01724-2, SOX17 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84936-1-PBS. Detection antibody: 84936-4-PBS. Standard: Ag19157. Range: 0.156-20 ng/mL.

    Cytometric bead array standard curve of MP01724-2

    Cytometric bead array standard curve of MP01724-2, SOX17 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84936-1-PBS. Detection antibody: 84936-4-PBS. Standard: Ag19157. Range: 0.156-20 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01724-2 targets SOX17 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.156-20 ng/mL (Cytometric Bead Array); 0.625-40 ng/mL (Sandwich ELISA);

Capture antibody: SOX17 Recombinant antibody, PBS Only (Capture) 84936-1-PBS (242515A6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: SOX17 Recombinant antibody, PBS Only (Detector) 84936-4-PBS (242515E11). 100 μg. Concentration 1 mgl/ml.

Alternative SOX17 matched antibody pairs: MP01724-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84936-1-PBS
Tested Reactivity human
Immunogen SOX17 fusion protein Ag19157
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SRY (sex determining region Y)-box 17
Synonyms Transcription factor SOX-17, Transcription factor SOX 17, 242515A6
GenBank accession numberBC111365
Gene symbol SOX17
UniprotQ9H6I2
Gene ID (NCBI) 64321
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 84936-4-PBS
Tested Reactivity human
Immunogen SOX17 fusion protein Ag19157
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SRY (sex determining region Y)-box 17
Synonyms Transcription factor SOX-17, Transcription factor SOX 17, 242515E11
GenBank accession numberBC111365
Gene symbol SOX17
UniprotQ9H6I2
Gene ID (NCBI) 64321
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C